Investigation of Optical Properties and Activity of Wheat Stripe Rust Urediospores

Stripe rust is one of the most common diseases challenging safe production wheat. Rapid identification and analysis urediospores, responsible for disease transmission, are key to preventing controlling stripe rust. The current spore detection time-consuming requires auxiliary equipment, but many optical methods sensors with high efficiency, sensitivity, ability have been developed widely used. Thus, investigation parameters prerequisite sensitive detailed in this study. Firstly, microscopic images surface elements wheat urediospores were obtained using a high-resolution electron microscope an energy spectrum analyzer. reflectivity absorptivity spectra near-infrared band active/inactivate measured. Further, parameters, such as reflection phase shift, complex refractive index, dielectric constant, analyzed based on electromagnetic theory Kramers–Kronig relation. results revealed that strong tolerance light, real part index n was between 1.0 1.4. These observations indicate whether spores active or not has little effect constant significant impact imaginary extinction coefficient corresponding relationship properties biological components established. findings verified HPLC-MS Near-Infrared Spectroscopy Analysis Technology lay theoretical foundation detecting by sensors. study provides reference characteristics other samples.